New aziridine derivatives and their preparation methods

ABSTRACT

The present invention relates to new aziridine derivative that is represented by following general chemical formulae (la) or (lb), and to their preparation method. In the above mentioned chemical formulae, R 2  and R 3  can be same or different, and they are hydrogen, low-quality alkyl or cycloalkyl respectively; R 4  can be selected among hydrogen, alkyl, aril, or amino protective group, and amino protective group is, for example, (PH) 3 C, FMOC (9-fluorenylmethyl oxycarbonyl), alkoxycarbonyl, ariloxycarbonyl, aralkyloxycarbonyl and R 5 CO, R 5 SO 2  where R 5  is alkyl or aril or aralkyl.

TECHNICAL FIELD

The present invention relates to the preparation methods of aziridine derivatives, and in particular to the preparation methods of aziridine derivatives which are important intermediates for the synthesis of oligopeptide analogues including HIV (Human Immunodeficiency Virus) protease inhibitors.

BACKGROUND ART

The HIV is a retrovirus with RNA-type genetic information. As the medical treatment agents of the virus, there are reverse transcriptase inhibitors and HIV protease inhibitors, etc. However, since almost all the inhibitors are only capable of preventing an infection of cell but not capable of preventing the virus duplication in an infected cell, the inhibitors are generally known as an inhibitor for extending a life span of human slightly rather than curing the disease. Due to the development of a certain virus having tolerance to the compounds, a new curative medicine with a new structure is urgently needed.

The HIV protease inhibitors developed so far are Saquinavir of the Roche company(European Patent 432695 A(1991)) Amprenavir of the Glaxo-Wellcome company(U.S. Pat. No. 941982(1992)), Indinavir of the Merck company(U.S. Pat. No. 789508(1991)), Ritronavir of the Abbott company(US Patent No. 998114(1992)), and Nelfinavir of the Agouron company(U.S. Pat. No. 5,484,926(1996)). The compounds are mainly used for treating or preventing the acquired immune deficiency syndrome(AIDS) due to HIV infection.

The HIV protease inhibitors are belonging to an inhibitor referred as a hydroxyethylamine family. The compounds except for Nelfinavir, i.e., Saquinavir, Palinavir and Amprenavir have a benzyl group at the second carbon position in the structure of hydroxyethylamine.

As the intermediates for the synthesis of the HIV protease inhibitor, (2R)-[1′(S)-azido-2-phenylethyl]oxiran(J. Med. Chem. 1993, 36, 292-294). 3(S)-amino-1,2(S)-epoxy-4-phenylbutane, etc. have been known. Since the intermediates are prepared from phenylalanine, they always have benzyl groups at the structure of hydroxyethylamin(HEA). However Nelfinavir has a phenylthiomethyl group instead of a benzyl at HEA, a intermediates with different structures such as 3(S)-amino-1,2(S)-epoxy-4-phenylbutane is necessary.

Therefore, in order to develop a conventional or new HIV protease inhibitor, new intermediates capable of accepting a benzyl group as well as phenylthiomethyl or other substituents are necessary.

DISCLOUSRE OF INVENTION

Accordingly, it is an object of the present invention to provide aziridine derivatives capable of accepting various substituents.

It is another object of the present invention to provide the preparation methods of crucial intermediates for the synthesis of HIV protease.

The present invention relates to aziridine derivatives of the following formula(Ia) or (Ib),

where R₂ and R₃ may be same or different and respectively represents hydrogen, low alkyl or cycloalkyl, and R₄ is selected from the group comprising a hydrogen, alkyl, aryl, aralkyl and amino protecting group. The amino protecting group is R₅CO or R₅SO₂, where R₅ is selected from the group comprising alkyl, aryl and aralkyl; CH₃—Ph—SO₂—, (Ph)₃C, FMOC(9-fluorenylmethyloxycarbonyl), alkoxycarbonyl, aryloxycarbonyl or araklyloxycarbonyl, preferably t-butoxycarbonyl or benzyloxycarbonyl, and their preparation methods. According to the invention, the derivatives of aziridine with amino group and hydroxy group protected from ascorbic acid, can be prepared. The aziridine derivatives are useful and important intermediates for preparing HIV protease inhibitors.

A preparation method for aziridine derivatives with formula (Ia) and (Ib) is described as follows:

-   -   (a) The epoxy compound of the formula (1a) or (1b) where R₂ and         R₃ may be same or different and respectively represents hydrogen         or low alkyl group, are reacted with NaN₃ for thereby preparing         a 1-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol         derivative of formula (2a) or (2b) where R₂ and R₃ are same as         defined above; and     -   (b) the compound of the formula (2a) or (2b) where R₂ and R₃ are         same as defined above, are formed to compounds which have an         amine ring structure and being protected by an amino protection         group, to prepare aziridine derivatives of the formula (Ia) or         (Ib) where R₂, R₃ and R₄ are same as defined above.

The following chemical reaction formula(Ia) illustrates an example of the above method .

[chemical reaction formula 1a]

The following chemical reaction formula(Ib) illustrates another example of the above method

[chemical reaction formula 1b]

The epoxy compounds of formula (1a) or (1b) can be prepared by the known methods using ascorbic acid as a starting material. The methods are disclosed in J. Org. Chem. 1990, Vol 55, 4400-4403; chem.. Soc. Perkin Trans. 1 1995 1783-1785; Tetrahedron Letters 1990, Vol31, No7. 1003-1006; J. Org. Chem. 1988, Vol 53, 2598-2602, etc.

For example, the epoxy compound of formula (1a) may be prepared using a L-ascorbic acid as a starting material, and the epoxy compound of (1b) may be prepared from a D-Isoascorbic acid.

As a solvent, methanol or ethanol/NH₄Cl, methylformate, etc. can be used in the step(a) and acetonitrile, toluene, THF, DMF, etc. can be used in the step(b).

The present invention provides the preparation methods for aziridine derivatives of the following formula(II):

where R₁ represents phenyl or phenyl substituted with halogen, alkyl, alkoxy, ROCOCH₂O— in which R represents alkyl, or benzyloxy group; phenylthio or alkylthio; heteroaryl with more than one nitrogen, oxygen or sulfur atom; cycloalkyl or cycloalkyl substituted with alkyl group; alkyl or alkenyl with C₁-C₄ carbon atoms; alkenyl substituted with a phenyl or OC₄H₆N—(CH₂)₂—Ph—; heterocycle including a nitrogen and/or oxygen; and heterocycle including a nitrogen and/or oxygen with an alkyl group,

which are important intermediates for synthesizing HIV protease using an aziridine derivative of the following formula(I),

where R₂, R₃ and R₄ have the same definitions as above.

The compound of formula(II) is prepared from an aziridine derivative of formula(I) by the following method.

(a) The compound of the following formula(6) where R₁, R₂, R₃ and R₄ have the same definitions as above is prepared by adding R₁ to the compound of formula(I),

(b) the compound of the following formula(7) where R₁, R₂, R₃ and R₄ have the same definitions as above is prepared by removing a hydroxyl protecting group from the compound of formula(6), and

(c) an aziridine derivative of the following formula(II) is prepared by epoxidation of the compound of the following formula(7) with the above R₁, R₂, R₃ and R_(4.)

The following chemical reaction formula(II) illustrates the preparation method for the compound of formula(II) which is started from the compound of formula(I).

[Chemical reaction formula II]

Formula(I) represents the compound of formula(Ia) or formula(Ib). It is possible to prepare various isomers by adjusting the reaction conditions for preparing the compound of formula(II) from the compound of formula(I).

For example, in the case that R₁ is phenyl group among the compounds of formula(II), the compound can be a raw material for synthesizing the Amprenavir. In addition, streoisomers which can be used as a PI intermediate of the BMS Co. can be prepared by controlling streochemistry selectively in the step(c) in which the compound of formula(II) where R₁ is phenyl group is prepared from the compound of the formula(7).

In addition, in the case that R₁ is a phenylthio group, the compound is a synthesizing material of Nelfinavir.

BEST MODE FOR CARRYING OUT THE INVENTION

Now, the invention will be described in detail by the examples.

I. Preparation of an Aziridine Derivative Of the Formula(I) EXAMPLE 1 (2R,3S)1-azido-2-hydroxy-3 ,4-isopropylidenebutane-2,3 4-triol(2a)

(2S,3R)3,4-epoxy- 1,2-O-isopropylidenebutane-1,2-diol(32.9g) was dissolved in the mixture of 410 ml of ethanol and 40 ml of water. After 85.4 ml of methyl formate was added to the solution, 40 ml of water and 36.9 g of NaN₃ were added to the solution. The solution was stirred at 50˜60° C. for 6 hours. At the end of the reaction, ethanol was distillated under reduced pressure, and the resultant solution was diluted with 300 ml of water. The resultant solution was extracted using 300 ml of methylene chloride. After the solution was treated with anhydrous Na₂SO₄ and distillated under reduced pressure, a light yellow liquid compound of (2R,3S)l-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol was obtained (40.6g, yield: 95%).

1H-NMR(300 MHz, CDCl₃); δ1.35(s,3H), 1.42(s, 3H), 2.83(d,1H), 3.40(dd,1H), 3.53(dd,1H), 3.71˜3.78(m,1H), 3.93˜4.10(m,1H)

EXAMPLE 2 (2R,3S)1,2-O-isopropylidene-3,4-(t-butoxycarbonyl(imino-butane-1,2-diol(Ia)

(2R,3S)l-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol(40.6 g) was dissolved in 800 ml of acetonitrile and PPh₃ of 56.9 g(1 equivalent) was added to the solution. The solution was stirred at a room temperature for 1 hour and refluxed for 12 hours at an increased temperature. The solution was cooled down to room temperature and distillated under reduced pressure. 200 ml of 1,4-dioxane and 200 ml of water were added to the solution and 36.4 g of NaHCO₃(2 equivalents) was added to the solution. Thereafter, (Boc) ₂O of 50.4 ml(1 equivalent) was added. After the resultant solution was stirred at room temperature for 1 hour, the solvent was distillated under reduced pressure. And then the solution was diluted with 250 ml of diethyl ether and was washed with 250 ml of water. The separated ether layer was treated with Na₂SO₄ anhydride and distillated under reduced pressure. A white liquid compound of (2R,3S)-1,2-O -isopropylidene-3,4-(t-butoxycarbonyl)imino-butane-1,2-diol was obtained(50 g, yield: 95%).

[α]_(D) ²⁵⁼⁻72.19(Cl, CHCl3)

1H-NMR(300 MHz, CDCl₃): δ1.35(s,3H), 1.44(s,3H), 1.46(s,9H), 2.13(d,1H), 2.28(d,1H), 2.51˜2.56(m,1H), 3.84˜3.87(m,1H), 4.03˜4.07(m,2H)

EXAMPLE 3 (2R,3R)1-azido-2-hroxy-3,4-isopropylidenebutane-2,3,4-triol(2b)

(2R,3R)-3,4-epoxy- 1,2-O-isopropylidenebutane-1,2-diol(32.9 g) was dissolved in the mixture of 410 ml of ethanol and 40 ml of water. 85.4 ml of methyl formate was added to the solution and then 40 ml of water and 36.9 g of NaN₃ were added to the solution. The solution was stirred at 50˜60° C. for 6 hours. At the end of the reaction, the ethanol was distillated under reduced pressure and the solution was diluted with 300 ml of water and was extracted three times with 300 ml of methylene chloride. The resultant solution was treated with Na₂SO₄ anhydride and distillated under reduced pressure. A light yellow liquid compound of (2S,3S)1-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol(2b) was obtained(40.7 g, yield: 95%).

EXAMPLE 4 (2S,3S)1,2-O-isopropylidene-3,4-(t-butoxycarbonyl)imino-butane-1,2-diol(Ib)

(2S,3S)1-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol(40.6 g) was dissolved in 800 ml of acetonitrile and 56.9 g(1 equivalent) of PPh₃ was added to the solution. After the solution was stirred at room temperature for 1 hour, it was stirred with reflux at an increased temperature for 12 hours. The solution was cooled down to room temperature and the solvent was distillated under reduced pressure. 200 ml of 1,4-dioxane and 200 ml of water were added to the resultant. 36.4 g(2 equivalents) of NaHCO₃ and (Boc)₂O of 50.4 ml(1 equivalent) were added to the solution respectively. After the solution was stirred at room temperature for 1 hour, the solvent was distillated under reduced pressure. The resultant was diluted with 250 ml of diethyl ether and washed with 250 ml of water. The separated ether layer was treated with Na₂SO₄ anhydride and then distillated under reduced pressure. The titled compound of (2S ,3S)1,2-O-isopropylidene-3,4-(t-butoxycarbonyl)imino-butane-1,2-diol was obtained as a white liquid(50.1 g, yield: 95%).

[α]_(D) ²⁵⁼⁻45.14(Cl, CHCl₃) 1H-NMR(300 MHz, CDCl₃): α1.35(s,3H), 1.45(s,9H), 1.46(s,3H), 2.14(d,1H), 2.35(d,1H), 2.47˜2.52(m,1H), 3.74˜3.80(m,1H), 3.98˜4.03(m,1H), 4.16˜4.21(m,1H)

II. Synthesis of Aziridine Derivative of Formula(II) EXAMPLE 5 (2R,3S)-1,2-O-isopropylidene-3-(t-butoxycarbonyl)-amino-4-phenylbutane(6)

The mixture of (2R,3S)-1,2-O-isopropylidene-3,4-(t-butoxycarbonyl)imino-butane-1,2-diol(50 g) and 308 ml of toluene was cooled down to −10° C. in the presence of N₂ and CuBr.Sme₂ (2.1 g) and 154 ml of PhMgCl(2M in THF) was added to the mixture respectively in the temperature of below −10° C. After the mixture was stirred in the temperature of below −10° C. for 1 hour, it was added to 308 ml of NH₄Cl solution(33 g of NH₄Cl) and extracted by 300 ml of toluene. A white solid compound of (2R,3S)-1,2-O-isopropylidene-3-(t-butoxycarbonyl)-amino-4-phenylbutane was obtained after distillation under reduced pressure.

1H-NMR(300 MHz, CDCl₃): δ1.33(s,3H), 1.40(s,9H), 1.47(s,3H), 2.79˜2.96(m,2H), 3.65(dt,1H), 3.82˜3.93(m,2H), 4.09(dt,1H), 4.82(d,1H), 7.19˜7.33(m,1H)

EXAMPLE 6 (2R,3S)-3-(t-butoxycarbonyl)amino-4-phenyl-butane-1,2-diol(7)

(2R,3S)- 1,2-O-isopropylidene-3-(t-butoxycarbonyl)-amino-4-phenylbutane(20 g) was dissolved in the mixture of 180 ml of methanol and 200 ml of water and 0.6 g(0.05 equivalent) of p-TsOH was added to the solution. The solution nvas stirred at 50° C. for 6 hours. After the solution was neutralized by adding 0.9 g(0.1 equivalent) of K₂CO₃, it was distillated under reduced pressure. The resultant was diluted with 200 ml of water, extracted twice by 200 ml of EtOAc, treated with Na₂SO₄ anhydride and distillated under reduced pressure. The titled compound of (2R,3S)-3-(t-butoxycarbonyl)amino-4-phenyl- butane-1,2-diol was obtained.

EXAMPLE 7 (2S,3S)-3-(t-butoxycarbonyl)amino-1,2-epoxy-4-phenylbutane(II)

(2R,3S)-3-(t-butoxycarbonyl)amino-4-phenoyl-butane- 1,2-diol(21.8 g) was dissolved in 150 ml of EtOAc and cooled down to below 10° C. 6.9 ml(1.1 equivalent) of pyridine and 9 ml (1 equivalent) of benzoyl chloride were added to the solution and it was stirred at 5˜10° C. for 2 hours. After addition of 7.2 ml(1.2 equivalent) of Ms-Cl, 21.6 ml(2 equivalents) of triethyl amine was added to the solution in the temperature of below 15° C. and it was stirred for 2 hours. The solution was washed by 150 ml of 1N HCl, 150 ml of 5% aq.NAHCO₃ and 150 ml of water and distillated under reduced pressure. 150 ml of 1.4-dioxane was added to the resultant and after the addition of 85 ml of 2M KOH, it Aas stirred at room temperature for 2 hours. After 150 ml of water was added to the solution, it was extracted by 150 ml of toluene and the toluene layer was washed by 150 ml of water. The layer was distillated under reduced pressure and the resultant was recrystallized in IPA/H₂O. A white crystal compound of (2S,3S)-3-(t-butoxycarbonyl)amino-1,2-epoxy-4-phenylbutane was obtained(mp: 125.6° C.).

[α]_(D) ²³=8.18(Cl, MeOH)

1H-NMR(300 MHz, CDCl₃): δ1.39(s,9H) 2.75˜3.05(m,5H), 3.71(broad s, 1H), 4.50(broad s, 1H), 7.2˜7.38(m,5H)

The above compound is intermediate for Amprenavir of the Glaxo-Wellcome.

EXAMPLE 8 (2R,3S)-3-(t-butoxycarbonyl)amino- 1,2-epoxy-4-phenylbutane(II)

To the mixture of (2R,3S)-3-(t-butoxycarbonyl)amino-4-phenyl-butane-1,2-diol(44 g) and 400 ml of pyridine, 60 g(2 equivalents) of p-TsCl was added and the solution was stirred at 0° C. for 4 hours. After pH of the solution was adjusted 10 with 25% NaOH and it was stirred at 0° C. for 3 hours. 400 ml of methylene chloride was added to the solution and it was washed by 400 ml of IN HCl, 400 ml of 5% aq.NaHCO₃ and 400 ml of water and was distillated under reduced pressure. A white crystal compound of (2R,3S)-3-(t-butoxycarbonyl)amino-1,2-epoxy-4-phenylbutane was obtained using a column chromatography.

1H-NMR(300 MHz, CDCl₃): δ1.37(s,9H), 2.55˜3.02(m,5H), 4.10(s,1H), 4.40(s, 1H), 7.16˜7.35(m,5H)

The above compound is the PI intermediate of the BMS company.

EXAMPLE 9 (1R)- 1-benzyloxycarbonylamino-1-[(4R)-2,2-dimethyl -1,3-dioxalane-4-il]2-phenylthioethane(6)

13.3 ml(1.6 equivalent) of PhSH was drop-added to 5.2 g(1.6 equivalent) of NaH in 200 ml of THF at 0° C. under N₂ atmosphere.

After the generation of H₂, was stopped, the solution was stirred at room temperature for 30 minutes. The mixture of (2R,3S)-1,2-O-isopropylidene-3,4-(benzyloxycarbonyl)imino-butane-1,2-diol(22.5 g) and 100 ml of THF was slowly drop-added to the solution at room temperature. After the resultant solution was stirred at room temperature for 5 hours, 200 ml of water was added and followed by removal of THF and extraction by 200 ml of ether. The solution was treated wvith Na₂SO₄ anhydride and concentrated under reduced pressure. A crude compound of (1R)-1-benzyloxycarbonylamnino-1-[(4R)-2,2-dimethyl- 1,3-dioxalane-4-il]2-phenyltioethane was obtained.

EXAMPLE 10 (2R,3R)-3-benzyloxycarbonylamino-4-phenylthio-1,2-butanediol(7)

The crude compound of (2R,3R)-3-benzyloxycarbonylamino-1-[(4R)-2,2-dimethyl-1,3-dioxalane-4-il]-2-phenylthioethane in the example 9 was dissolved in 200 ml of methanol and 100 of 1N HCl was added to the solution. The solution was stirred at 5° C. for 2 hours, concentrated under reduced pressure and extracted by 200 ml of ethyl acetate. The resultant solution was treated with Na₂SO₄ anhydride and concentrated under reduced pressure. A crude state compound of (2R,3R)-3-benzyloxycarbonylamino-4-phenyltio-1,2-butanediol was obtained.

EXAMPLE 11 (2S,3R)-3-benzyloxycarbonylamino-4-phenylthio-1-buteneoxide(II)

After the crude compound of (2R,3R)-3-benzyloxycarbonylamino-4-phenylthio-1,2-butanediol(13.3 g) from the example 10 was dissolved in 110 ml of EtOAc, 4 ml (1.3 equivalent) of pyridine and 5.3 ml (1.2 equivalent) of benzoyl chloride were added to the solution and it was stirred at 5˜10° C. for 5 hours. 3.6 ml(1.2 equivalent) of Ms-Cl was added to the solution and 12.8 ml(2.4 equivalents) of triethyl amine was drop-added in the temperature of below 15° C. After the addition, it was stirred for 30 minutes. 1.5 ml(1 equivalent) of methanol was added to the solution and stirred at room temperature for 1 hour. The resultant solution was rinsed using 90 ml of 1N HCl, 90 ml of 5% NaHCO₃ and 50 ml of H₂O and distillated under reduced pressure. To the concentrated solution, 180 ml of 1.4-dioxane was added and the solution of 5.56 g(2.2 equivalent) of 85% KOH in 42 ml of H₂O was added. The resultant solution was stirred at room temperature for 2 hours. The solution was diluted with 280 ml of water and extracted using 280 ml of toluene. After the toluene layer was rinsed by 170 ml of H₂O, 170 ml of 5% NaHCO₃ and 170 ml of brine and treated with Na₂SO₄ anhydride, it was filtered and concentrated under reduced pressure. A white crystal compound of (2 S,3R)-3-benzyloxycarbonylamino-4-phenylthio-1-buteneoxide was obtained by column chromatography using Hexane: EtOAc=2:1. mp 61.7° C.

[α]_(D) ²⁵=25.4(cl, CHCl₃)

1H-NMR(300 MHz, CDCl₃): δ2.70˜2.80(m,2H), 3.00(m,1H), 3.22(d,2H), 3.70(m,1H), 5.08(s,2H), 5.10(d,1H), 7.10˜7.20(m,10H)

The above compound is Nelfinavir of the Agouron company.

As described above, the new aziridine derivatives according to the invention can be used as crucial intermediates for the synthesis of Amprenavir and Nelfinavir among the HIV protease inhibitors. (2S,3S)-3-(t-butoxycarbonyl)amino-1,2-epoxy-4-phenylbutane which is a crucial intermediate for the synthesis of Amprenavir can be prepared by accepting phenyl group. By accepting a phenylthio group instead of the phenyl group, (2S,3R)-benzyloxycarbonylamino-4-phenylthio-1-buteneoxide also can be prepared. The compound is a crucial intermediate for the synthesis of Nelfinavir. From the streoisomer of the compound, (2S,3S)-3-(t-butoxycarbonyl)amino-1,2-epoxy-4-phenylbutane, the synthesis of a HIV protease inhibitor with different structure with Amprenavir is possible . 

1. Aziridine derivatives of the following formula (Ia) or (Ib)

where R₂ and R₃ may be the same or different and, respectively represent hydrogen, low alkyl or cycloalkyl, and R₄ is selected from the group comprising hydrogen, alkyl, aryl, aralkyl and amino protecting group.
 2. The compound of claim 1, wherein said amino protecting group is selected from the group comprising a t-butoxycarbonyl, benzyloxycarbonyl, CH₃—Ph—SO ₂—; (Ph)₃C, and FMOC(9-fluorenylmethyloxycarbonyl).
 3. The compound of claim 1, wherein said R4 represents a t-butoxycarbonyl.
 4. A method for preparing aziridine derivatives of the following formula (Ia) or (Ib)

where R₂ and R₃ may be the same or different and respectively represent hydrogen, low alkyl or cycloalkyl, and R4 is selected from the group comprising hydrogen, alkyl, aryl, aralkyl and amino protecting group, which is comprised of steps (a) step of reacting the epoxy compound of formula (la) or (lb) in which R₂ and R₃ may be same or different and respectively represent hydrogen or low alkyl group, with NaN₃ to prepare 1-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol derivatives of formula (2a) or (2b) in which R₂ and R₃ are same as defined above; and

(b) step of forming amino ring structure of said compound of formula (2a) or (2b) and protecting by the amino protecting group to prepare said compound of formula (Ia) or (Ib).
 5. The method of claim 4, wherein said amino protecting group is selected from the group comprising a t-butoxycarbonyl, benzyloxycarbonyl, CH ₃—Ph—SO₂—; (Ph)₃C, and FMOC(9-fluorenylmethyloxycarbonyl).
 6. A method for preparing aziridine derivatives of the following formula (II)

where R₁ represents phenyl or phenyl substituted with halogen, alkyl, alkoxy, ROCOCH₂O— in which R represents alkyl, or benzyloxy group; phenylthio or alkylthio; heteroaryl with more than one nitrogen, oxygen or sulfur atom; cycloalkyl or cycloalkyl substituted with alkyl group; alkyl or alkenyl with C₁-C₄ carbon atoms; alkenyl substituted with a phenyl or OC₄H₆N—(CH₂)₂—Ph—; heterocycle including a nitrogen and/or oxygen; and heterocycle including a nitrogen and/or oxygen with an alkyl group and R4 is selected from the group comprising hydrogen, alkyl, aryl, aralkyl and amino protecting group, which is comprised of steps (a) adding said R₁ to the compound of the following formula (I)

where R₂ and R₃ may be the same or different and respectively represent hydrogen, low alkyl or cycloalkyl, and R4 is selected from the group comprising hydrogen, alkyl, aryl, aralkyl and amino protecting group, to prepare the compound of the following formula (6)

where R₁, R₂, R₃ and R4 are same as defined above, removing hydroxyl protecting group of said compound of formula (6) to prepare the compound of the following formula (7)

where R₁ and R4 are the same as defined above, and (b) epoxidation of said compound of formula (7) to prepare the said compound of formula (II).
 7. The method of claim 6, wherein said R₁ represents a phenyl or phenylthio group.
 8. The method of claim 6, wherein said compound of the formula (I) is prepared by the method which is comprised of steps (a) step of reacting the epoxy compound of formula (la) or (lb) in which R₂ and R₃ may be the same or different and respectively represent hydrogen or low alkyl group, with NaN₃ to prepare 1-azido-2-hydroxy-3,4-isopropylidenebutane-2,3,4-triol derivatives of formula (2a) or (2b) in which R₂ and R₃ are the same as defined above; and

(b) step of forming amino ring structure of said compound of said compound of formula (2a) or (2b) and protecting group to prepare said compound of formula (I).
 9. The compound of claim 2, wherein said R4 represents a t-butoxycarbonyl. 